Green Synthesis of Silver Nanoparticles from Flower Extract of Hibiscus rosa-sinensis and Its Antibacterial Activity

Silver nanoparticles (SNPs) exhibit magnificent applications in medicine as antimicrobial agent. The use of different plant parts for the synthesis of nanoparticles is considered as a green technology. This technology is one of the best eco-friendly method and does not gives any harmful effect. The present study deals with the flower extract of medicinal plant Hibiscus rosa-sinensis and to evaluate its anti-bacterial properties. The synthesis and characterization of silver nanoparticles was confirmed by UV-Visible spectrophotometer, Fourier Transform Infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM). Agar well diffusion method was used to confirm the antibacterial activity of silver nanoparticles. UV-Visible absorption spectra of the reaction medium containing silver nanoparticles showed maximum absorbance at 422 nm. FTIR analysis confirmed reduction of Ag+ ions to Ag0 ions in synthesized silver nanoparticles. The SEM analysis showed the particle size between 5-40 nm and spherical in structure. The silver nanoparticles have shown bactericidal effects against Aeromonas hydrophila infected fish Catla catla. This study revealed that H.rosa-sinensis synthesized silver nanoparticles exhibit good and potent antimicrobial activity against fish pathogen.